Organ Perfusion Technology
”At Reno-NL, we are dedicated to advance biomedical research and industry-driven innovations by developing and applying organ perfusion protocols using surplus organs from slaughterhouses and, when appropriate, human organs. We are convinced that this approach offers a more ethical, cost-effective and translatable alternative to traditional preclinical models, bridging the gap between research and human clinical applications.”
Organ perfusion is a preclinical screening model that can offer added value for your kidney research. This dynamic ex vivo model is characterized by the original organ anatomy and physiology, such as intact kidney architecture, vascularization, and metabolism. Combined with real-time monitoring and precise control of perfusion parameters during experimentation, the model provides more accurate and superior results than traditional in vitro models. Furthermore, using organs from slaughterhouse animals that exhibit high translatability to human situation, it also reduces the need for traditional laboratory animal experimentation, aligning with the 3R principles.
Organ perfusion process
Organ retrieval
The process begins with controlled removal of the organ. For our preclinical studies, we use slaughterhouse-sourced organs. Rapid and careful retrieval ensures optimal preservation and the best condition for the next phase.
Cold perfusion
Immediately after retrieval, the organ is connected to our cold perfusion system. A cold preservation solution keeps the cells viable and minimizes damage. This step is critical for maintaining organ quality.
Warm perfusion
Next, we mimic body conditions through a stage of warm perfusion. Oxygen and nutrients are circulated to recondition the organ. This phase also allows tracking of kidney health, and real-time monitoring of its response to various biomedical treatments.
Analysis
In the final stage, we process all samples collected during the study and perform high-end analyses of both liquid samples (perfusate and urine) and tissue biopsies. This includes assessment of organ viability, function, disease-specific markers, microscopy, pathway analysis, and pharmacokinetic/ toxicological profiling.
About our technology
Reno-NL perfusion protocol: Therapeutics or medical devices can be tested at various stages in our process. The process is flexible and can be adjusted to the research question. Combining cold (HMP = hypothermic machine perfusion) with warm (NMP = normothermic machine perfusion) phases allows long-term treatments and sampling at various conditions.
Our NMP circuit: Perfusions of kidney tissue are performed under controlled conditions similar to human physiology (37°C, oxygenation, mean arterial pressure 50-70 mm Hg, and well-balanced perfusate composition)
Figures are created in https://BioRender.com
